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KMID : 0616619990050010063
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Journal of Soonchunhyang Medical College
1999 Volume.5 No. 1 p.63 ~ p.72
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Expression of p53 and bcl-2 in Epidermal Tumors of the Skin
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Abstract
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Background :
The function of p53 protein was known to accelerate the apoptosis, but bcl-2 protein was known to suppress the apoptosis. The overexpression of p53 and bcl-2 proteins has been reported in many malignant tumors. Although the mechanism of tumorgenesis of the p53 and bcl-2 protein took hasnot been proved, bcl-2 positive tumors contrast to p53, appeared to have good prognosis.
The expression pattern of p53 and bcl-2 in epidermal tumors has not been well known.
The author analyzed immunohistochemical expression of p53 and bcl-2 protein and
investigated the role of p53 and bcl-2 protein in the developing tumors of epidermal origin.
Method :
15 cases of squamous cell carcinoma, 20 cases of basal cell carcinoma, 10 cases of keratoacanthoma and 6 cases of Bowen´s disease were stained immunohistochemically stain with anti p53 and anti bcl-2 monoclonal antibody.
Results :
1. In squamous cell carcinoma, the reaction of p53 was positive in 13 cases(86%) and the stain pattern showed more intensive reaction in poorly differentiated cells in the same tumor.
bcl-2 showed negative in all cases of squamous cell carcinoma(0%).
2. In basal cell carcinoma, the reaction of p53 was positive in 10 cases (50%).
The positive cells were mainly observed in squamous metaplastic portion, but the reaction was weak.
bcl-2 showed positive in all cases of basal cell carcinoma (100%) and the reaction was strong.
3. In keratoacanthoma, p53 was positive in 7 cases (70%).
The positive cells were mainly observed in the cells around the basement membrane.
This finding suggested that p53 positive basal cells were programmed to become
keratoacanthoma.
bcl-2 was negative in all 10 cases of keratoacanthoma. (0%) In some cases, bcl-2 positive cells were observed around the basement membrane in some cases., which were negative to p53 This finding suggested that the existence of these bcl-2 positive cells were related to the prognosis of keratoacanthoma.
4. In Bowen´s disease, p53 was positive in 5 cases ( 83% ) and the reaction was
strong, so the tumor cells in Bowen´s disease appeared to be poorly differentiated type.
bcl-2 was negative in all 6 cases ( 0% ) of Bowen´s disease, but bcl-2 positive cells were observed in the basement membrane in some cases.
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